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CRISPR-Cas: A Laboratory Manual by Jennifer Doudna

• CRISPR-Cas: A Laboratory Manual
• Jennifer Doudna
• Page: 450
• Format: pdf, ePub, mobi, fb2
• ISBN: 9781621821311
• Publisher: Cold Spring Harbor Laboratory Press

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CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

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CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells  Crispr-Cas: A Laboratory Manual: Amazon.de: Jennifer Doudna
The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign DNA, these powerful techniques  CRISPR Systems - Santa Cruz Biotechnology
CRISPR Cas9 dCas9 gene editing plasmids for gene knockout and gene Lab Supplies The CRISPR/Cas system is an adaptive immune defense mechanism used by Archea and bacteria for the degradation of foreign genetic material. Mouse Genome Editing using CRISPR/Cas System
The CRISPR/Cas9 components (sgRNA, Cas9 mRNA with or without a repair DNA) Manipulating the mouse embryo: a laboratory manual. Patent US8945839 - CRISPR-Cas systems and methods for altering
the CRISPR-Cas system comprises one or more nuclear Fritsch and Maniatis , MOLECULAR CLONING: A LABORATORY MANUAL, 2nd  "Mouse Genome Editing Using the CRISPR/Cas System - InnoSer
Keywords: CRISPR/Cas9 r sgRNA r gene editing r mutant mouse r pronuclear and cytoplasmic the Mouse Embryo: A Laboratory Manual. 4th Edition. Cold Spring Harbor Lab Press Books
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. CRISPR-Cas – Revolution from the lab 1. Introduction - COGEM
1. CRISPR-Cas – Revolution from the lab. COGEM Report and advice CGM/ 141030-01. 1. Introduction. In recent years great strides forward have been made   Genome engineering using the CRISPR-Cas9 - Zhang Lab @ MIT
and the RNA-guided CRISPR-Cas nuclease system18–25. The first two technologies use the Illumina user manual. Off-target indel analysis  Crispr/Cas: A Laboratory Manual von University Jennifer Doudna
Crispr/Cas: A Laboratory Manual von University Jennifer Doudna, Prashant Mali ( ISBN 978-1-62182-130-4) versandkostenfrei vorbestellen. Lieferung direkt  Patent EP2764103B1 - Crispr-cas systems and methods for altering
An engineered, non-naturally occurring Type II CRISPR-Cas vector and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL,  Cold Spring Harbor Lab Press Books
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.

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